IgM-antibody responses of chickens to salivary antigens of Triatoma infestans as early biomarkers for low-level infestation of triatomines

0352618 - BC 2011 RIV AU eng J - Journal Article
Schwarz, Alexandra - Medrano-Mercado, N. - Billingsley, P. F. - Schaub, G. A. - Sternberg, J. M.
IgM-antibody responses of chickens to salivary antigens of Triatoma infestans as early biomarkers for low-level infestation of triatomines.
International Journal for Parasitology. Roč. 40, č. 11 (2010), s. 1295-1302. ISSN 0020-7519. E-ISSN 1879-0135
Institutional research plan: CEZ:AV0Z60220518
Keywords : antibody response * Chagas disease * chickens * IgM * recombinant protein * salivary proteins * surveillance * Triatoma infestans
Subject RIV: EC - Immunology
Impact factor: 3.822, year: 2010

The recombinant form of a highly immunogenic 14.6 kDa protein in Triatoma infestans saliva (rTiSP14.6) is a potential epidemiological marker for the detection of triatomine bug populations using IgG responses in peridomestic chickens. However, the persistence of the IgG response prevents it being of value for several months in areas where triatomine control programmes have been implemented. In this investigation, IgM-antibody reactions to crude salivary antigens or rTiSP14.6 decayed rapidly after exposure of chickens and were measurable for only 18 days after a single challenge with T. infestans. In serial exposure experiments, chickens from low and high exposure groups showed no significant differences in anti-saliva and anti-rTiSP14.6 IgM-antibody titres. Highly immunogenic salivary antigens of 12 and 14 kDa were recognised by all chicken sera.
Permanent Link: http://hdl.handle.net/11104/0192089