Multilocus sequence analysis of Borrelia bissettii strains from North America reveals a new Borrelia species, Borrelia kurtenbachii

0352314 - BC 2011 RIV DE eng J - Journal Article
Margos, G. - Hojgaard, A. - Lane, R. S. - Cornet, M. - Fingerle, V. - Rudenko, Natalia - Aanensen, D. M. - Fish, D. - Piesman, J.
Multilocus sequence analysis of Borrelia bissettii strains from North America reveals a new Borrelia species, Borrelia kurtenbachii.
Ticks and Tick-borne Diseases. Roč. 1, č. 4 (2010), s. 151-158. ISSN 1877-959X. E-ISSN 1877-9603
Institutional research plan: CEZ:AV0Z60220518
Keywords : Borrelia bissettii * Borrelia kurtenbachii sp. nov. * Ixodes * Multilocus sequence analysis * Molecular ecology
Subject RIV: EC - Immunology
Impact factor: 0.000, year: 2010

Using multilocus sequence analysis (MLSA), we investigated the phylogenetic relationship of spirochaete strains from North America previously assigned to the genospecies Borrelia bissettii.We amplified internal fragments of 8 housekeeping genes (clpA, clpX, nifS, pepX, pyrG, recG, rplB, and uvrA) located on the main linear chromosome by polymerase chain reaction. Phylogenetic analysis of concatenated sequences of the 8 loci showed that the B. bissettii clade consisted of 4 closely related clusters which included strains from California and Colorado that were isolated from Ixodes pacificus, I. spinipalpis, or infected reservoir hosts. Several strains isolated from I. scapularis clustered distantly from B. bissettii. Genetic distance analyses confirmed that these strains are more distant to B. bissettii than B. carolinensis, a recently described Borrelia species, which suggests that they constitute a new Borrelia genospecies, Borrelia kurtenbachii.
Permanent Link: http://hdl.handle.net/11104/0191847