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Raman spectroscopy for the characterization of algal cells

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    0352210 - UPT-D 2011 RIV US eng C - Conference Paper (international conference)
    Samek, Ota - Jonáš, Alexandr - Pilát, Zdeněk - Zemánek, Pavel - Nedbal, Ladislav - Tříska, Jan - Kotas, Petr - Trtílek, M.
    Raman spectroscopy for the characterization of algal cells.
    17th Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics (Proceedings of SPIE Vol. 7746). Bellingham: SPIE, 2010, 77460X: 1-7. ISBN 978-0-8194-8236-5.
    [Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics /17./. Liptovsky Jan (SK), 06.09.2010]
    R&D Projects: GA MŠk ED0017/01/01; GA MŠk OC08034
    Grant - others:EC(XE) PERG 06-GA-2009-256526
    Institutional research plan: CEZ:AV0Z20650511; CEZ:AV0Z60870520
    Keywords : Raman spectroscopy * algal cells * lipids * iodine value
    Subject RIV: BH - Optics, Masers, Lasers

    Raman spectroscopy can elucidate fundamental questions about intercellular variability and what governs it. Moreover, knowing the metabolic response on single cell level this can significantly contribute to the study and use of microalgae in systems biology and biofuel technology. Raman spectroscopy is capable to measure nutrient dynamics and metabolism in vivo, in real-time, label free making it possible to monitor/evaluate population variability. Also, degree of unsaturation of the algae oil (iodine value) can be measured using Raman spectra obtained from single microalgae. The iodine value is the determination of the amount of unsaturation contained in fatty acids (in the form of double bonds). Here we demonstrate the capacity of the spatially resolved Raman microspectroscopy to determine the effective iodine value in lipid storage bodies of individual living algal cells.
    Permanent Link: http://hdl.handle.net/11104/0191777