Regulation of the Chlamydomonas Cell Cycle by a Stable, Chromatin-Associated Retinoblastoma Tumor Suppressor Complex

0351683 - MBÚ 2011 RIV US eng J - Journal Article
Olson, B. J. S. C. - Oberholzer, M. - Li, Y. - Zones, J. M. - Kohli, H. S. - Bišová, Kateřina - Fang, S. - Ch. - Meisenhelder, J. - Hunter, T. - Umen, J. G.
Regulation of the Chlamydomonas Cell Cycle by a Stable, Chromatin-Associated Retinoblastoma Tumor Suppressor Complex.
Plant Cell. Roč. 22, č. 10 (2010), s. 3331-3347. ISSN 1040-4651. E-ISSN 1532-298X
Institutional research plan: CEZ:AV0Z50200510
Keywords : GENOME-WIDE ANALYSIS * TRANSCRIPTION FACTOR * GENE-PRODUCT
Subject RIV: EE - Microbiology, Virology
Impact factor: 9.396, year: 2010

We examined the cell cycle dynamics of the retinoblastoma (RB) protein complex in the unicellular alga Chlamydomonas reinhardtiithat has single homologs for each subunit—RB, E2F, and DP. We found that Chlamydomonas RB (encoded by MAT3) is a cellcycle–regulated phosphoprotein, that E2F1-DP1 can bind to a consensus E2F site, and that all three proteins interact in vivo to form a complex that can be quantitatively immunopurified. Yeast two-hybrid assays revealed the formation of a ternary complex between MAT3, DP1, and E2F1 that requires a C-terminal motif in E2F1 analogous to the RB binding domain of plant and animal E2Fs
Permanent Link: http://hdl.handle.net/11104/0191386