Involvement of carotenoids in the synthesis and assembly of protein subunits of photosynthetic reaction centers of Synechocystis sp. PCC 6803

0351636 - MBÚ 2011 RIV JP eng J - Journal Article
Sozer, O. - Komenda, Josef - Ughy, B. - Domonkos, I. - Laczkó-Dobos, H. - Malec, P. - Gombos, Z. - Kis, M.
Involvement of carotenoids in the synthesis and assembly of protein subunits of photosynthetic reaction centers of Synechocystis sp. PCC 6803.
Plant and Cell Physiology. Roč. 51, č. 5 (2010), s. 823-835. ISSN 0032-0781. E-ISSN 1471-9053
R&D Projects: GA AV ČR IAA400200801
Institutional research plan: CEZ:AV0Z50200510
Keywords : Carotenoidless mutant * crtB * Membrane protein synthesis
Subject RIV: EE - Microbiology, Virology
Impact factor: 4.257, year: 2010

The crtB gene of Synechocystis sp. PCC 6803, encoding phytoene synthase, was inactivated in the Delta crtH mutant to generate a carotenoidless Delta crtH/B double mutant. Delta crtH mutant cells were used because they had better transformability than wild-type cells, most probably due to their adaptation to partial carotenoid deficiency. Cells of the Delta crtH/B mutant were light sensitive and could grow only under light-activated heterotrophic growth conditions in the presence of glucose. Carotenoid deficiency did not significantly affect the cellular content of phycobiliproteins while the chlorophyll content of the mutant cells decreased
Permanent Link: http://hdl.handle.net/11104/0191346