Role of FtsH2 in the repair of Photosystem II in mutants of the cyanobacterium Synechocystis PCC 6803 with impaired assembly or stability of the CaMn4 cluster

0351624 - MBÚ 2011 RIV NL eng J - Journal Article
Komenda, Josef - Knoppová, Jana - Krynická, Vendula - Nixon, P. J. - Tichý, Martin
Role of FtsH2 in the repair of Photosystem II in mutants of the cyanobacterium Synechocystis PCC 6803 with impaired assembly or stability of the CaMn4 cluster.
Biochimica Et Biophysica Acta-Bioenergetics. Roč. 1797, č. 5 (2010), s. 566-575. ISSN 0005-2728. E-ISSN 1879-2650
R&D Projects: GA AV ČR IAA400200801
Institutional research plan: CEZ:AV0Z50200510
Keywords : CtpA protease * D1 degradation and maturation * FtsH protease
Subject RIV: EE - Microbiology, Virology
Impact factor: 5.132, year: 2010

The FtsH2 protease, encoded by the slr0228 gene, plays a key role in the selective degradation of photodamaged D1 protein during the repair of Photosystem II (PSII) in the cyanobacterium Synechocystis sp. PCC 6803. To test whether additional proteases might be involved in D1 degradation during high rates of photodamage, we have studied the synthesis and degradation of the D1 protein in DPsbO and DPsbV mutants, in which the CaMn4 cluster catalyzing oxygen evolution is less stable, and in the D1 processing mutants, D1-S345P and DCtpA, which are unable to assemble a functional cluster. All four mutants exhibited a dramatically increased rate of D1 degradation in high light compared to the wild type. Additional inactivation of the ftsH2 gene slowed the rate of D1 degradation dramatically and increased the level of PSII complexes. We conclude that FtsH2 plays a major role in the degradation of both precursor and mature forms of D1 following donor-side photoinhibition
Permanent Link: http://hdl.handle.net/11104/0191336