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Access to enantiopure aromatic epoxides and diols using epoxide hzdrolases derived from total biofilter DNA
- 1.0351499 - MBÚ 2011 RIV NL eng J - Journal Article
Kotík, Michael - Štěpánek, Václav - Grulich, Michal - Kyslík, Pavel - Archelas, A.
Access to enantiopure aromatic epoxides and diols using epoxide hzdrolases derived from total biofilter DNA.
Journal of Molecular Catalysis B-Enzymatic. Roč. 65, 1-4 (2010), s. 41-48. ISSN 1381-1177
R&D Projects: GA ČR GA204/06/0458
Institutional research plan: CEZ:AV0Z50200510
Keywords : Chiral resolution * Enantioconvergent reaction * Enantioselectivity
Subject RIV: CE - Biochemistry
Impact factor: 2.330, year: 2010
Two genes encoding functional epoxide hydrolases were retrieved from total DNA isolated from biofilter-derived biomass, using PCR with epoxide hydrolase-specific degenerate primers followed by genome-walking PCR. The resulting recombinant epoxide hydrolases were expressed in Escherichia coli and characterised
Permanent Link: http://hdl.handle.net/11104/0191238
Number of the records: 1