High-performance liquid chromatography–off line mass spectrometry analysis of anthraquinones produced by Geosmithia lavendula

0351441 - MBÚ 2011 RIV NL eng J - Journal Article
Stodůlková, Eva - Man, Petr - Kolařík, Miroslav - Flieger, Miroslav
High-performance liquid chromatography–off line mass spectrometry analysis of anthraquinones produced by Geosmithia lavendula.
Journal of Chromatography A. Roč. 1217, č. 40 (2010), s. 6296-6302. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA MŠMT 2B08064; GA AV ČR KAN200200651
Institutional research plan: CEZ:AV0Z50200510
Keywords : High-performance liquid chromatography * Mass spectrometry * Anthraquinones
Subject RIV: EE - Microbiology, Virology
Impact factor: 4.194, year: 2010

Lilac coloured species of Geosmithia lavendula produce a mixture of polyhydroxylated anthraquinones under condition of submerged fermentation. Three pigments had been isolated and identified earlier as a 1,3,6,8-tetrahydroxyanthraquinone (compound 7), rhodolamprometrin (1-acetyl 2,4,5,7-tetrahydroxyanthraquinone; compound 5), and 1-acetyl 2,4,5,7,8-penthahydroxyanthraquinone (compound 4). A new HPLC method was developed for the separation of three known and ten new anthraquinone pigments. In addition, five new pigments were determined by FTMS as coeluting impurities. The analyses were performed on a reversed phase column using gradient elution with a mobile phase system consisting of phosphate buffer (50mM, pH 2.0) and acetonitrile. The structure evaluation was based namely on FTMS and UV–VIS spectrometry. The developed procedure was used for the determination of individual anthraquinones in fermentation broth of G. lavendula after 14 days of cultivation
Permanent Link: http://hdl.handle.net/11104/0191196