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Effective Removal of Nonionic Detergents in Protein Mass Spectrometry, Hydrogen/Deuterium Exchange, and Proteomics
- 1.0351177 - MBÚ 2011 RIV US eng J - Journal Article
Rey, M. - Mrázek, H. - Pompach, Petr - Novák, P. - Pelosi, L. - Brandolin, G. - Forest, E. - Havlíček, Vladimír - Man, P.
Effective Removal of Nonionic Detergents in Protein Mass Spectrometry, Hydrogen/Deuterium Exchange, and Proteomics.
Analytical Chemistry. Roč. 82, č. 12 (2010), s. 5107-5116. ISSN 0003-2700. E-ISSN 1520-6882
R&D Projects: GA AV ČR KJB500200612; GA MŠMT LC545
Institutional research plan: CEZ:AV0Z50200510
Keywords : mass spectrometry * proteomics * peptides
Subject RIV: CE - Biochemistry
Impact factor: 5.874, year: 2010
Paper describes novel method suitable for removal of non-ionic POE-based detergents and PEG or PPG polymers. The removal is done as solid phase extraction with the peptides bound to the reversed phase resin. The detergent or polymer molecules are washed away using chlorinated solvents. Several aspects of the method were carefully tested and its sensitivity was shown. It is suitable for removal of different non-ionic detergents or polymers on peptide or protein level and it can be easily automated. Its compatibility with hydrogen/deuterium exchange was shown and demonstrated on the mapping of full membrane protein
Permanent Link: http://hdl.handle.net/11104/0190984
Number of the records: 1