Functional molecular analysis of a circadian clock gene timeless promoter from the drosophilid fly Chymomyza costata

0351175 - BC 2011 RIV US eng J - Journal Article
Kobelková, Alena - Bajgar, Adam - Doležel, David
Functional molecular analysis of a circadian clock gene timeless promoter from the drosophilid fly Chymomyza costata.
Journal of Biological Rhythms. Roč. 25, č. 6 (2010), s. 399-409. ISSN 0748-7304. E-ISSN 1552-4531
R&D Projects: GA ČR GP204/08/P579; GA ČR(CZ) GA204/07/1032; GA AV ČR IAA500960802
Institutional research plan: CEZ:AV0Z50070508
Keywords : timeless promoter * E-box * Chymomyza costata
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.309, year: 2010

The tim promoter from D. melanogaster has been studied in detail both in tissue cultures and in living flies yet has never been investigated in other species. This article presents a detailed functional analysis of the tim promoter from the drosophilid fly, Chymomyza costata, in Drosophila tissue cultures. A comparison of tim promoters from wt and npd-mutants confirmed that the 1855 bp deletion in the latter removes crucial regulatory cis-elements as well as the minimal promoter, being subsequently responsible for the lack of tim mRNA expression. Deletion and substitution mutations of the wt tim promoter showed that the region containing the canonical E-box, TER-box, and 2 incomplete E-box sequences is essential for CLK/CYC-mediated expression, while the PERR element appears to be a repressor in S2 cells. Furthermore, the expression of the circadian genes timeless, period, vrille, and doubletime was quantified in C. costata adults.
Permanent Link: http://hdl.handle.net/11104/0190982