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Isolation and characterization of novel pI 4.8 MnP isoenzyme from white-rot fungus Irpex lacteus
- 1.0351168 - MBÚ 2011 RIV US eng J - Journal Article
Sklenář, Jan - Niku-Paavola, M. - L. - Santos, S. - Man, Petr - Kruus, K. - Novotný, Čeněk
Isolation and characterization of novel pI 4.8 MnP isoenzyme from white-rot fungus Irpex lacteus.
Enzyme and Microbial Technology. Roč. 46, č. 7 (2010), s. 550-556. ISSN 0141-0229. E-ISSN 1879-0909
R&D Projects: GA AV ČR IAAX00200901; GA ČR GP310/08/P258; GA MŠMT LC06066
Institutional research plan: CEZ:AV0Z50200510
Keywords : Solid-support growth * White-rot fungus * Irpex lacteus
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.287, year: 2010
This paper desribes optimalization of the growth conditions of white-rot fungus Irpex lacteus, a potential candidate for use in bioremediation in situ, on polyurethane solid support. The main objective was isolation and characterization of manganese-dependent peroxidase (MnP) isoenzyme (pI 4.8) exhibitng the highest activity. Optimization of the enzyme production and isolation procedure covering anion exchange chromatography (Mono Q) was performed. The purified protein was subjected to LC-MSMS characterization which provided information on the amino acid sequence as well as the glycosylation
Permanent Link: http://hdl.handle.net/11104/0190976
Number of the records: 1