The Arabidopsis Exocyst Complex Is Involved in Cytokinesis and Cell Plate Maturation

0351017 - ÚEB 2011 RIV US eng J - Journal Article
Fendrych, Matyáš - Synek, Lukáš - Pečenková, Tamara - Toupalová, Hana - Cole, R. - Drdová, Edita - Nebesářová, Jana - Šedinová, M. - Hála, Michal - Fowler, J.E. - Žárský, Viktor
The Arabidopsis Exocyst Complex Is Involved in Cytokinesis and Cell Plate Maturation.
Plant Cell. Roč. 22, č. 9 (2010), s. 3053-3065. ISSN 1040-4651. E-ISSN 1532-298X
R&D Projects: GA MŠMT(CZ) LC06034; GA AV ČR KJB600380806; GA AV ČR KJB600380802
Grant - others:GA MŠk ME10033
Institutional research plan: CEZ:AV0Z50380511; CEZ:AV0Z60220518
Keywords : ELECTRON TOMOGRAPHIC ANALYSIS * SACCHAROMYCES-CEREVISIAE * PLANT TRANSFORMATION
Subject RIV: ED - Physiology
Impact factor: 9.396, year: 2010

Secretory pathways play a crucial role in cytokinesis in plants from the onset of phragmoplast initiation to the maturation of the cell plate. Tethering of secretory vesicles at the plasma membrane is mediated by the exocyst complex. Using proteomic and cytologic approaches, we show that EXO84b is a subunit of the plant exocyst. A. thaliana mutants for EXO84b are severely dwarfed and have compromised leaf epidermal cell and guard cell division. During cytokinesis, green fluorescent protein– tagged exocyst subunits SEC6, SEC8, SEC15b, EXO70A1, and EXO84b exhibit distinctive localization maxima at cell plate initiation and cell plate maturation, stages with a high demand for vesicle fusion. Finally, we present data indicating a defect in cell plate assembly in the exo70A1 mutant. We conclude that the exocyst complex is involved in secretory processes during cytokinesis in Arabidopsis cells, notably in cell plate initiation, cell plate maturation, and formation of new primary cell wall.
Permanent Link: http://hdl.handle.net/11104/0190861