Isolation and characterization of two new lipopeptide biosurfactants produced by Pseudomonas fluorescens BD5 isolated from water from the Arctic Archipelago of Svalbard

0351013 - MBÚ 2011 RIV NL eng J - Journal Article
Janek, T. - Lukaszewicz, M. - Řezanka, Tomáš - Krasowska, A.
Isolation and characterization of two new lipopeptide biosurfactants produced by Pseudomonas fluorescens BD5 isolated from water from the Arctic Archipelago of Svalbard.
Bioresource Technology. Roč. 101, č. 15 (2010), s. 6118-6123. ISSN 0960-8524. E-ISSN 1873-2976
Institutional research plan: CEZ:AV0Z50200510
Keywords : Pseudomonas fluorescens BD5 * Lipopeptide * Pseudofactins
Subject RIV: EE - Microbiology, Virology
Impact factor: 4.365, year: 2010

The arctic freshwater bacterium Pseudomonas fluorescens BD5 produces biosurfactants when grown on 2 percent glucose. Crude biosurfactants were extracted from a cell-free culture supernatant with ethyl acetate and purified by preparative reversed phase high performance liquid chromatography (RP-HPLC). The chemical structure of the purified biosurfactants, pseudofactin I and II, was analyzed by matrix assisted laser desorption/ionization time of flight (MALDI TOF) mass spectrometry and tandem mass spectrometry (MS/MS). Both compounds are novel cyclic lipopeptides with a palmitic acid connected to the terminal amino group of eighth amino acid in peptide moiety
Permanent Link: http://hdl.handle.net/11104/0190858