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RP-HPLC/MS-APCI analysis of odd-chain TAGs from Rhodococcus erythropolis including some regioisomers
- 1.0351012 - MBÚ 2011 RIV IE eng J - Journal Article
Řezanka, Tomáš - Schreiberová, O. - Krulikovská, T. - Masák, J. - Sigler, Karel
RP-HPLC/MS-APCI analysis of odd-chain TAGs from Rhodococcus erythropolis including some regioisomers.
Chemistry and Physics of Lipids. Roč. 163, 4-5 (2010), s. 373-380. ISSN 0009-3084. E-ISSN 1873-2941
R&D Projects: GA MŠMT 2B08062
Institutional research plan: CEZ:AV0Z50200510
Keywords : Rhodococcus erythropolis * RP-HPLC/MS-APCI * Odd-chain triacylglycerols
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.861, year: 2010
We used reversed phase liquid chromatography–atmospheric pressure chemical ionization mass spectrometry (RP-HPLC/MS-APCI) for direct analysis of triacylglycerols (TAGs) from four different cultivations of Rhodococcus erythropolis CCM 2595. This technique enabled us to identify and quantify the specific molecular species of TAGs directly from lipid extracts of the bacterium, including the determination of their basic characteristics such as retention time and mass spectra. A total of 17 TAGs having at least one odd-numbered-chain FA (fatty acid) were found. This is the first time when odd-chain TAGs, predominantly with pentadecanoic, margaric, and margaroleic acids, were identified
Permanent Link: http://hdl.handle.net/11104/0190857
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