A dual surface plasmon resonance assay for the determination of ribonuclease H activity

0350621 - ÚFE 2011 RIV GB eng J - Journal Article
Šípová, Hana - Vaisocherová, Hana - Štepánek, J. - Homola, Jiří
A dual surface plasmon resonance assay for the determination of ribonuclease H activity.
Biosensors and Bioelectronics. Roč. 26, č. 4 (2010), s. 1605-1611. ISSN 0956-5663. E-ISSN 1873-4235
R&D Projects: GA AV ČR KAN200670701; GA MŠMT OC09058; GA ČR GA202/09/0193
Grant - others:Univerzita Karlova(CZ) SVV-2010-261 304
Institutional research plan: CEZ:AV0Z20670512
Keywords : Surface plasmon resonance * Enzyme activity assay * Ribonuclease H * Biosensor
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering
Impact factor: 5.361, year: 2010

We report on a new assay based on surface plasmon resonance (SPR) which allows for simultaneous 1) real-time monitoring of RNase H activity and 2) detection of cleavage reaction products. The potential of the assay is demonstrated in the study in which the ability of natural and modified oligonucleotides to activate RNase H is examined.
Permanent Link: http://hdl.handle.net/11104/0190575