New in vitro method for evaluating antiviral activity of acyclic nucleoside phosphonates against plant viruses

0350494 - BC 2011 RIV NL eng J - Journal Article
Špak, Josef - Holý, Antonín - Pavingerová, Daniela - Votruba, Ivan - Špaková, Vlastimila - Petrzik, Karel
New in vitro method for evaluating antiviral activity of acyclic nucleoside phosphonates against plant viruses.
Antiviral Research. Roč. 88, č. 3 (2010), s. 296-303. ISSN 0166-3542. E-ISSN 1872-9096
R&D Projects: GA ČR GA522/09/0707
Institutional research plan: CEZ:AV0Z50510513; CEZ:AV0Z40550506
Keywords : Brassica * Chemotherapy * Turnip yellow mosaic virus * Ribavirin
Subject RIV: CE - Biochemistry
Impact factor: 4.439, year: 2010

A new method was developed for testing antiviral compounds against plant viruses based on rapidly growing brassicas in vitro on liquid medium. This method enables exchange of media containing tested chemicals in various concentrations and simultaneous evaluation of their phytotoxicity and antiviral activity. While using ribavirin as a standard for comparison, phytotoxicity and ability of the acyclic nucleotide analogues (R)-PMPA, PMEA, PMEDAP, and (S)-HPMPC to eliminate ssRNA Turnip yellow mosaic virus (TYMV) were evaluated by this method. Double antibody sandwich ELISA and real-time PCR were used for relative quantification of viral protein and nucleic acid in plants. Ribavirin had the most powerful antiviral effect against TYMV. On the other hand, (R)-PMPA and PMEA had no antiviral effect and almost no phytotoxicity compared to the control. (S)-HPMPC and PMEDAP showed moderate antiviral effect, accompanied by higher phytotoxicity.
Permanent Link: http://hdl.handle.net/11104/0190488