Specific cell cultivation on nanofibrous layer

0350418 - ÚMCH 2011 RIV CH eng J - Journal Article
Michálek, Jiří - Mareková, Dana - Martinová, L. - Hobzová, Radka - Kostecká, Petra - Lubasová, D. - Lesný, Petr - Přádný, Martin - Jendelová, Pavla - Syková, Eva
Specific cell cultivation on nanofibrous layer.
Advances in Science and Technology. Roč. 76, - (2010), s. 139-142. ISSN 1662-0356.
[International Conferences on Modern Materials & Technologies, Forum on New Materials /5./. Montecatini Terme, 13.06.2010-18.06.2010]
R&D Projects: GA ČR GA304/07/1129; GA AV ČR KJB400500902
Institutional research plan: CEZ:AV0Z40500505; CEZ:AV0Z50390703
Keywords : hepatocytes * hepatal bioreactor * bioartifical liver assist device
Subject RIV: CD - Macromolecular Chemistry

Nanofibrous layers of reproducible thickness and structure (Nanospider™ technology) were employed as carriers for hepatocytes and their efficiency were evaluated in a laboratory bioreactor. The construction of the functional hepatal bioreactor is particularly given by the positive response of hepatocyte cells to the used carrier layer as well as by the cell morphology, their viability and biological activity in certain period of time. We compared cell growth on collagen with nanofibres electrospun from copolymer (HEMA/EOEMA) and from differently prepared polycaprolactone (PCL) layers. In both cases, the cells showed better morphology or better growth the on controls (collagen). The results obtained in laboratory bioreactor proved the biochemical functionality of the studied system.
Permanent Link: http://hdl.handle.net/11104/0190431