New data on aetiology of nodular gill disease in rainbow trout, Oncorhynchus mykiss

0350371 - BC 2011 RIV CZ eng J - Journal Article
Dyková, Iva - Kostka, Martin - Wortberg, F. - Nardy, E. - Pecková, Hana
New data on aetiology of nodular gill disease in rainbow trout, Oncorhynchus mykiss.
Folia Parasitologica. Roč. 57, č. 3 (2010), s. 157-163. ISSN 0015-5683. E-ISSN 1803-6465
R&D Projects: GA ČR GA524/09/0137; GA MŠMT LC522
Institutional research plan: CEZ:AV0Z60220518
Keywords : nodular gill disease * aetiological study * amoebae * Naegleria sp. * fish diseases * aquaculture * Oncorhynchus mykiss
Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine
Impact factor: 1.533, year: 2010

We studied amoebae associated with nodular gill disease (NGD) outbreaks in rainbow trout Oncorhynchus on kiss (Walbaum) in fish farms in South-Western Germany. Gills of 12 diseased rainbow trout were examined in fresh, by isolation attempts, histologically and using in situ hybridisation (ISH). A total of nine amoeba strains of the genera Acanthamoeba (I), Hartmannella (2), Naegleria (1), Protacanthamoeba (1) and Vannella (4) were isolated and determined using light microscopical, ultrastructural and molecular methods. Specific molecular probes designed from the SSU rDNA sequences of individual amoeba strains were used for non-radioactive ISH in histological sections. Association of Naegleria sp. with NGD and a direct ISH proof of Naegleria trophozoites attached to hyperolastic gill epithelium are novel findings, expanding the number of possible agents of NGD and supporting the hypothesis on multicausal aetiology of this disease.
Permanent Link: http://hdl.handle.net/11104/0190389