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Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells

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    0350113 - ÚEM 2011 RIV GB eng J - Journal Article
    Kunová, M. - Matulka, K. - Eiselleová, L. - Trčková, P. - Hampl, Aleš - Dvořák, Petr
    Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells.
    Reproductive BioMedicine Online. Roč. 21, - (2010), s. 676-686. ISSN 1472-6483. E-ISSN 1472-6491
    Grant - others:GA MŠk(CZ) LC06077; EC FP6(XE) LSHG-CT-2006-018739
    Program: LC
    Institutional research plan: CEZ:AV0Z50390512; CEZ:AV0Z50390703
    Keywords : animal protein-free culture * high-density culture * human embryonic stem cells
    Subject RIV: EB - Genetics ; Molecular Biology
    Impact factor: 2.285, year: 2010
    http://arl-repository.lib.cas.cz/uloziste_av/UEM-P/cav_un_epca-0350113_01.pdf

    For human embryonic stem cells (hESC) to be used in cell replacement therapies, they must be grown under good manufacturing conditions in a chemically defined medium that lacks animal proteins. This study examined the ability of a newly designed medium containing the plant-derived serum replacement VegetaCell and other reagents of human origin to support undifferentiated growth and pluripotency of hESC. Even under the most stringent feeder-free conditions without conditioned medium, hESC exhibited an undifferentiated morphology, expressed markers of undifferentiated cells, demonstrated high alkaline phosphatase activity and multilineage differentiation and retained a normal karyotype.
    Permanent Link: http://hdl.handle.net/11104/0190197

     
     
Number of the records: 1  

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