Sustainable use of Genetic Diversity in Forage and Turf Breeding

0349995 - ÚEB 2011 RIV NL eng M - Monography Chapter
Kopecký, David - Bartoš, Jan - Lukaszewski, A.J. - Baird, J. H. - Černoch, V. - Kölliker, R. - Sandve, S.R. - Rognli, O.A. - Blois, H. - Caig, V. - Doležel, Jaroslav - Kilian, A.
DArTFest – A Platform for High-Throughput Genome Profiling Within the Festuca – Lolium Complex.
Sustainable use of Genetic Diversity in Forage and Turf Breeding. Dordrecht: Springer, 2010 - (Huyghe, C.), s. 443-448. ISBN 978-90-481-8705-8
R&D Projects: GA MZe QH71267; GA ČR GP521/07/P479
Institutional research plan: CEZ:AV0Z50380511
Keywords : DArT * Fescue * Hybrids
Subject RIV: EB - Genetics ; Molecular Biology
www.springerlink.com/content/j776m4/#section=713733&page=1 http://arl-repository.lib.cas.cz/uloziste_av/UEB-Q/cav_un_epca-0349995_01.pdf

With the aim to facilitate high-throughput genome profiling and genetic and physical mapping within the Festuca-Lolium, complex we have developed a Diversity Arrays Technology (DArT) array for five important species: Festuca pratensis, Festuca arundinacea, Festuca glaucescens, Inlium perenne and Lolium multiflorum. The DArTFest array contains 7,680 probes derived from methylfiltered genomic representations. Of 3,884 polymorphic DArT markers identified in the first marker discovery experiment, over 1,000 markers detected a positive allele in each species. We assigned DArT markers to individual chromosome regions of F. pratensis using a series of single chromosome substitution and recombinant lines of F. pratensis in L. multiflorum. Moreover, we enriched the existing genetic map of F. pratensis by over 200 DArT markers and existing genetic map of L. multiflorum by over 500 DART markers.
Permanent Link: http://hdl.handle.net/11104/0190105