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Infrared spectroscopy-based metabolomic analysis of maize growing under different nitrogen nutrition

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    0349941 - ÚEB 2011 RIV CZ eng J - Journal Article
    Pavlík, Milan - Pavlíková, D. - Vašíčková, Soňa
    Infrared spectroscopy-based metabolomic analysis of maize growing under different nitrogen nutrition.
    Plant, Soil and Environment. Roč. 56, č. 11 (2010), s. 533-540. ISSN 1214-1178. E-ISSN 1805-9368
    R&D Projects: GA MZe 1G58027; GA MŠMT 2B06024
    Institutional research plan: CEZ:AV0Z50380511; CEZ:AV0Z40550506
    Keywords : ammonium * ecdysone phosphate * CULTAN
    Subject RIV: GD - Fertilization, Irrigation, Soil Processing
    Impact factor: 1.076, year: 2010
    www.agriculturejournals.cz/publishedArticle?journal=PSE&volume=56&firstPage=533 http://arl-repository.lib.cas.cz/uloziste_av/UEB-Q/cav_un_epca-0349941_01.pdf

    For metabolomic analysis of maize plants growing under different nitrogen nutrition sequential extraction of fresh biomass was used and isolated fractions were characterized and evaluated using IR spectra. The IR spectra of individual fractions were evaluated in relation to the nitrogen rates (2 g or 4 g N), to applied fertilizers (ammonium nitrogen or urea ammonium nitrate solution) and sampling period. For butanol fraction, typical bands of flavonoids, polar phospholipids, steryl glycosides, analogues of ecdysteroids were characterized. The IR spectra of BuOH fraction showed changes of relative contents of isolated compounds mainly affected by nitrogen rates. For water fraction bands of organic acids, salts of organic acids, flavonoid glycosides and oligopeptides (phytochelatins and/or glutathione) were the most significant. The results showed an increased induction of oxalic acid in plants after 4 g N application.
    Permanent Link: http://hdl.handle.net/11104/0190062

     
     
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