The effect of arsenic contamination on amino acids metabolism in Spinacia oleracea L

0349939 - ÚEB 2011 RIV US eng J - Journal Article
Pavlík, Milan - Pavlíková, D. - Staszková, L. - Neuberg, M. - Kaliszová, R. - Száková, J. - Tlustoš, P.
The effect of arsenic contamination on amino acids metabolism in Spinacia oleracea L.
Ecotoxicology and Enviromental Safety. Roč. 73, č. 6 (2010), s. 1309-1313. ISSN 0147-6513. E-ISSN 1090-2414
R&D Projects: GA ČR GA521/09/1150
Institutional research plan: CEZ:AV0Z50380511
Keywords : Chronic stress * Delta(1)-pyrroline-5-carboxylate synthetase * Soil contamination
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 2.340, year: 2010

Changes of amino acid concentrations (proline, glutamate, asparagine, aspartate, alanine) and glutamate kinase activity (GKA) in plants under arsenic chronic stress reported here reveal their role in plant arsenic stress adaptation. Results of the pot experiment confirmed the toxic effect of arsenic at tested levels (As1=25, As2=50, As3=75 mg As kg-1 soil) for spinach. Growing available arsenic contents in soil were associated with the strong inhibition of above-ground biomass and with the enhancement of As plant content. The changes of glutamate, asparagine, aspartate and proline levels in the plants showed strong linear dependences on arsenic concentration in plants (R2=0.60-0.90). Compared to the untreated control, concentrations of free proline and aspartate of As3 treatment were enhanced up to 381 % and 162 %, respectively. The significant changes of glutamate were observed on As2 and As3 treatments (increased level up to 188, respectively 617 %).
Permanent Link: http://hdl.handle.net/11104/0190060