Rhodococcus fascians Impacts Plant Development Through the Dynamic Fas-Mediated Production of a Cytokinin Mix

0349831 - ÚEB 2011 RIV US eng J - Journal Article
Pertry, I. - Václavíková, Kateřina - Gemrotová, Markéta - Spíchal, Lukáš - Galuszka, Petr - Depuydt, S. - Temmerman, W. - Stes, E. - De Keyser, A. - Riefler, M. - Biondi, S. - Novák, Ondřej - Schmülling, T. - Strnad, Miroslav - Tarkowski, Petr - Holsters, M. - Vereecke, D.
Rhodococcus fascians Impacts Plant Development Through the Dynamic Fas-Mediated Production of a Cytokinin Mix.
Molecular Plant-Microbe Interactions. Roč. 23, č. 9 (2010), s. 1164-1174. ISSN 0894-0282. E-ISSN 1943-7706
R&D Projects: GA MŠMT(CZ) LC06034; GA ČR GD522/08/H003
Institutional research plan: CEZ:AV0Z50380511
Keywords : LEAFY GALL FORMATION * CORYNEBACTERIUM-FASCIANS * ENDOGENOUS CYTOKININS
Subject RIV: ED - Physiology
Impact factor: 4.010, year: 2010

The phytopathogenic actinomycete Rhodococcus fascians D188 relies mainly on the linear plasmid-encoded fas operon for its virulence. The bacteria secrete six cytokinin bases that synergistically redirect the developmental program of the plant to stimulate proliferation of young shoot tissue, thus establishing a leafy gall as a niche. A yeast-based cytokinin bioassay combined with cytokinin profiling of bacterial mutants revealed that the fas operon is essential for the enhanced production of isopentenyladenine, trans-zeatin, cis-zeatin, and the 2-methylthio derivatives of the zeatins. Cytokinin metabolite data and the demonstration of the enzymatic activities of FasD (isopentenyltransferase), FasE (cytokinin oxidase/dehydrogenase), and FasF (phosphoribohydrolase) led us to propose a pathway for the production of the cytokinin spectrum.
Permanent Link: http://hdl.handle.net/11104/0189971