Isolated chromosomes as a new and efficient source of DArT markers for the saturation of genetic maps

0349830 - ÚEB 2011 RIV DE eng J - Journal Article
Wenzl, P. - Suchánková, Pavla - Carling, J. - Šimková, Hana - Huttner, E. - Kubaláková, Marie - Sourdille, P. - Paul, E. - Feuillet, C. - Kilian, A. - Doležel, Jaroslav
Isolated chromosomes as a new and efficient source of DArT markers for the saturation of genetic maps.
Theoretical and Applied Genetics. Roč. 121, č. 3 (2010), s. 465-474. ISSN 0040-5752. E-ISSN 1432-2242
R&D Projects: GA MŠMT(CZ) LC06004
Institutional research plan: CEZ:AV0Z50380511
Keywords : WHEAT TRITICUM-AESTIVUM * ARRAYS TECHNOLOGY DART * HEXAPLOID WHEAT
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.264, year: 2010

We describe how the diversity arrays technology (DArT) can be coupled with chromosome sorting to increase the density of genetic maps in specific genome regions. Chromosome 3B and the short arm of chromosome 1B (1BS) of wheat were isolated by flow cytometric sorting and used to develop chromosome- and chromosome arm-enriched genotyping arrays containing 2,688 3B clones and 384 1BS clones. Linkage analysis showed that 553 of the 711 polymorphic 3B-derived markers (78%) mapped to chromosome 3B, and 59 of the 68 polymorphic 1BS-derived markers (87%) mapped to chromosome 1BS, confirming the efficiency of the chromosome-sorting approach. To demonstrate the potential for saturation of genetic maps, we constructed a consensus map of chromosome 3B using 19 mapping populations, including some that were genotyped with the 3B-enriched array. The 3B-derived DArT markers doubled the number of genetic loci covered.
Permanent Link: http://hdl.handle.net/11104/0189970