Nuclear Genome Size: Are We Getting Closer?

0349827 - ÚEB 2011 RIV US eng J - Journal Article
Doležel, Jaroslav - Greilhuber, J.
Nuclear Genome Size: Are We Getting Closer?
Cytometry. Part A. 77A, č. 7 (2010), s. 635-642. ISSN 1552-4922. E-ISSN 1552-4930
R&D Projects: GA MŠMT(CZ) LC06004
Institutional research plan: CEZ:AV0Z50380511
Keywords : cytometric techniques * reference standards * genome sequencing
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.749, year: 2010

Correct information on genome size is important in many areas of research For a long time, scientists have been struggling to understand the reason for the huge variation in eukaryotic genome size and its biological significance More recently, the knowledge on genome size has become important to structure genome sequencing projects as their scale and cost depend on genome size. Despite the fact that the first estimates of genome size in eukaryotes were made more than 50 years ago, we are still not quite sure about the exact genome size in practically all animal and plant species Moreover, different estimates continue to be published for the same species These discrepancies compromise data comparison and interpretation and point to methodological problems, which include standardization. This article assesses the current state of DNA reference standards for flow cytometry and the issues related to their calibration.
Permanent Link: http://hdl.handle.net/11104/0189967