Insights into the effect of soil pH on N.sub.2./sub.O and N.sub.2./sub. emissions and denitrifier community size and activity

0349812 - BC 2011 RIV US eng J - Journal Article
Čuhel, Jiří - Šimek, Miloslav - Laughlin, R.J. - Bru, D. - Chéneby, D. - Watson, C.J. - Philippot, L.
Insights into the effect of soil pH on N₂O and N₂ emissions and denitrifier community size and activity.
Applied and Environmental Microbiology. Roč. 76, č. 6 (2010), s. 1870-1878. ISSN 0099-2240. E-ISSN 1098-5336
R&D Projects: GA MŠMT MEB020726; GA MŠMT LC06066; GA AV ČR IAA600660605
Institutional research plan: CEZ:AV0Z60660521
Keywords : soil pH * N₂O and N₂ emissions * denitrifier community
Subject RIV: EH - Ecology, Behaviour
Impact factor: 3.778, year: 2010

The objective of this study was to investigate how changes in soil pH affect the N₂O and N₂ emissions, denitrification activity, and size of a denitrifier community. We established a field experiment, situated in a grassland area, which consisted of three treatments which were repeatedly amended with a KOH solution (alkaline soil), an H₂SO₄ solution (acidic soil), or water (natural pH soil) over 10 months. At the site, we determined field N₂O and N₂ emissions using the ¹⁵N gas flux method and collected soil samples for the measurement of potential denitrification activity and quantification of the size of the denitrifying community by quantitative PCR of the narG, napA, nirS, nirK, nosZ denitrification genes. Overall, our results indicate that soil pH is of importance in determining the nature of denitrification end products.
Permanent Link: http://hdl.handle.net/11104/0189955