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Utilization of the linear mode of MALDI-TOF mass spectrometry in the study of glycation during the malting process
- 1.0349615 - ÚIACH 2011 RIV GB eng J - Journal Article
Laštovičková, Markéta - Mazanec, Karel - Smětalová, Dagmar - Bobálová, Janette
Utilization of the linear mode of MALDI-TOF mass spectrometry in the study of glycation during the malting process.
Journal of the Institute of Brewing. Roč. 116, č. 3 (2010), s. 245-250. ISSN 0046-9750. E-ISSN 2050-0416
R&D Projects: GA MŠMT 1M0570
Institutional research plan: CEZ:AV0Z40310501
Keywords : barley * brewing * glycation
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 0.897, year: 2010
The analysis of intact proteins, extracted from the barley grain, the malt and the aliquots collected during malting and presented in this paper, show the feasibility of using the linear mode of MALDI-TOF MS to obtain a profile of the proteins present at all stages of the malting process. The best and the most reproducible results were obtained after a desalting procedure using Nanosep 3K and applying DHAP as a matrix for the MS measurement of the intact proteins.
Permanent Link: http://hdl.handle.net/11104/0189805
Number of the records: 1