Bacterial single-cell activities along the nutrient availability gradient in a canyon-shaped reservoir: a seasonal study

0349508 - BC 2011 RIV DE eng J - Journal Article
Horňák, Karel - Jezbera, Jan - Šimek, Karel
Bacterial single-cell activities along the nutrient availability gradient in a canyon-shaped reservoir: a seasonal study.
Aquatic Microbial Ecology. Roč. 60, č. 3 (2010), s. 215-225. ISSN 0948-3055. E-ISSN 1616-1564.
[Congress of the International Limnological Society /31./. Kapské Město, 15.08.2010-20.08.2010]
R&D Projects: GA ČR(CZ) GA206/08/0015; GA ČR(CZ) GAP504/10/1534
Institutional research plan: CEZ:AV0Z60170517
Keywords : reservoir * bacterial activity * leucine and glucose incorporation * HNA and LNA cells
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.089, year: 2010

We investigated the linkages between the composition and the activities of bacterioplankton assemblages in three stations (Dam, Middle, River) in the meso-eutrophic Římov reservoir (Czech Republic). At all stations, Betaproteobacteria (BET) or Actinobacteria (ACT) dominated the bacterial community composition, while Cytophaga-Flavobacteria (CF) accounted for smaller proportions. ACT showed high proportions of cells incorporating both leucine and glucose in all samples, whereas only small fractions of CF were scored positive for the incorporation of these substrates. BET incorporated leucine preferentially to glucose in all samples. Interestingly, CF and BET significantly contributed to the pool of high nucleic acid cells (HNA) at Stn River, whereas the opposite was found for ACT, forming the pool of low nucleic acid cells (LNA) at all stations. Since ACT efficiently incorporated organic substrates, the LNA fraction represented a highly active component of bacterial assemblages.
Permanent Link: http://hdl.handle.net/11104/0189725