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Development of Chromosome-Specific BAC Resources for Genomics of Bread Wheat

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    0349181 - ÚEB 2011 RIV CH eng J - Journal Article
    Šafář, Jan - Šimková, Hana - Kubaláková, Marie - Čihalíková, Jarmila - Suchánková, Pavla - Bartoš, Jan - Doležel, Jaroslav
    Development of Chromosome-Specific BAC Resources for Genomics of Bread Wheat.
    Cytogenetic and Genome Research. Roč. 129, 1-3 (2010), s. 211-223. ISSN 1424-8581. E-ISSN 1424-859X
    R&D Projects: GA ČR GA521/07/1573; GA MŠMT(CZ) LC06004
    Grant - others:European Community’s Seventh Framework Programme(XE) FP7/2007–2013
    Institutional research plan: CEZ:AV0Z50380511
    Keywords : BAC library * Chromosome * DNA markers
    Subject RIV: GE - Plant Breeding
    Impact factor: 1.783, year: 2010

    The large bread wheat genome (1C similar to 17 Gbp) contains a preponderance of repetitive DNA and the species is polyploid. These characteristics together serve to hamper the molecular analysis of the wheat genome. Its complexity can, however, be reduced by using flow cytometry to isolate individual chromosomes, and these can be exploited to construct chromosome-specific BAC libraries. Such libraries simplify the task of physical map construction, positional cloning and the targeted development of genetic markers. Rapid improvements in the efficiency and cost of DNA sequencing provide an opportunity to contemplate sequencing the wheat genome by preparing sequence-ready physical maps for each chromosome or chromosome arm in turn. The quality of the chromosome-specific libraries depends on their chromosome coverage and the mean insert size. First-generation libraries suffered from a relatively low mean insert size.
    Permanent Link: http://hdl.handle.net/11104/0189489

     
     
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