Multi-component polymeric system for tumour cell-specific gene delivery using a universal bungarotoxin linker

0349126 - ÚMCH 2011 RIV US eng J - Journal Article
Willemsen, R. A. - Pechar, Michal - Carlisle, R. C. - Schooten, E. - Pola, Robert - Thompson, A. J. - Seymour, L. W. - Ulbrich, Karel
Multi-component polymeric system for tumour cell-specific gene delivery using a universal bungarotoxin linker.
Pharmaceutical Research. Roč. 27, č. 11 (2010), s. 2274-2282. ISSN 0724-8741. E-ISSN 1573-904X
R&D Projects: GA ČR GA203/08/0543; GA AV ČR KAN200200651
Institutional research plan: CEZ:AV0Z40500505
Keywords : adenovirus * hydrophilic polymers * tumour targeting
Subject RIV: CD - Macromolecular Chemistry
Impact factor: 4.456, year: 2010

A new universal tool for specific, non-covalent and non-destructive attachment of a recombinant antibody fragment to a polymer-modified adenovirus has been utilised to regulate the tropism of adenoviral gene delivery vector. We have prepared a multivalent reactive N-(2-hydroxypropyl)methacrylamide-based copolymer (PHPMA) bearing an alpha-bungarotoxin-binding peptide (BTXbp). The copolymer was used for covalent surface modification of adenoviral vectors (Ad). A single chain variable fragment of anti-PSMA antibody bearing bungarotoxin (scFv-BTX) binding to the prostate-specific membrane antigen (PSMA) was conjugated with the copolymer-coated Ad. The presented Ad-PHPMA-BTXbp/scFv-BTX system can be used as a universal tool for a receptor-specific virotherapy.
Permanent Link: http://hdl.handle.net/11104/0006077