Dynamic saturation optical microscopy: employing dark-state formation kinetics for resolution enhancement

0349037 - ÚFCH JH 2011 RIV GB eng J - Journal Article
Humpolíčková, Jana - Benda, Aleš - Macháň, Radek - Enderlein, J. - Hof, Martin
Dynamic saturation optical microscopy: employing dark-state formation kinetics for resolution enhancement.
Physical Chemistry Chemical Physics. Roč. 12, č. 39 (2010), s. 12457-12465. ISSN 1463-9076. E-ISSN 1463-9084
R&D Projects: GA MŠMT(CZ) LC06063; GA AV ČR KJB400400904; GA AV ČR GEMEM/09/E006
Institutional research plan: CEZ:AV0Z40400503
Keywords : fluorescence microscopy * dunamic saturation optical microscopy * fluorescence
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 3.454, year: 2010

Fluorescence microscopy has become one of the most rapidly developing observation techniques in the field of molecular biology, since its high sensitivity, contrast and labeling specificity together with being non-invasive fulfill the most important requirements of live cell imaging. The biggest limitation of the technique seems to be the spatial resolution which is, based on Abbe's diffraction law, restricted to some hundreds of nanometres. Recently, various approaches have been developed that overcome the limit imposed by the diffraction barrier and these methods currently lead the development in the field of fluorescence microscopy. In this contribution, we present dynamic saturation optical microscopy (DSOM) a new technique that monitors the temporal decay of the excited singlet state due to a dark state formation. By mapping the intensity dependent decay kinetics, enhanced resolution images can be obtained.
Permanent Link: http://hdl.handle.net/11104/0189381