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Magnetic affinity separation of recombinant fusion proteins
- 1.0348877 - ÚVGZ 2011 RIV TR eng J - Journal Article
Šafařík, Ivo - Šafaříková, Miroslava
Magnetic affinity separation of recombinant fusion proteins.
Hacettepe Journal of Biology and Chemistry. Roč. 38, č. 1 (2010), s. 1-7. ISSN 1303-5002
R&D Projects: GA MŠMT(CZ) OC 157; GA MPO(CZ) 2A-1TP1/094
Institutional research plan: CEZ:AV0Z60870520
Keywords : recombinant fusion proteins * affinity tags * magnetic separation
Subject RIV: CE - Biochemistry
Isolation and separation of proteins is an important procedure in all areas of biosciences and biotechnology. Currently recombinant fusion proteins are produced routinely; in order to simplify their isolation from complex samples, magnetic separation techniques can be efficiently used. This short review paper shows examples of the most important fusion affinity tags and commercially available magnetic affinity and ion exchange particles enabling efficient capture of recombinant fusion proteins. A list of realized isolation procedures documents the efficiency of magnetic separation techniques.
Permanent Link: http://hdl.handle.net/11104/0189282
Number of the records: 1