Immunofluorescent labeling of tissues and free cells via QD-protein conjugates

0348649 - ÚIACH 2012 RIV CZ eng C - Conference Paper (international conference)
Lišková, Marcela - Voráčová, Ivona - Hezinová, Věra - Klepárník, Karel - Foret, František
Immunofluorescent labeling of tissues and free cells via QD-protein conjugates.
NANOCON 2010. 2nd International Conference. Ostrava: Tanger, 2011, s. 203-208. ISBN 978-80-87294-19-2.
[NANOCON 2010. International Conference /2./. Olomouc (CZ), 12.10.2010-14.10.2010]
R&D Projects: GA AV ČR KAN400310651; GA ČR GA203/08/1680; GA MŠMT LC06023
Institutional research plan: CEZ:AV0Z40310501
Keywords : quantum dots * cell labeling * conjugation
Subject RIV: CB - Analytical Chemistry, Separation
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Preparation of quantum dots and using their QD-protein conjugates in immunofluorescent labeling of cells and tissues. For conjugation was used method viaEDC/sulfo-NHS zero-length cross-linkers. We linked QD with annexin V, anti-CD3 and anti-PCNA. We labeled T-lymphocytes, apoptotic lymphocyte, mouse duodenum tissue and tumor tissue.
Permanent Link: http://hdl.handle.net/11104/0189107