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RP-HPLC/MS-APCI Analysis of Branched Chain TAG Prepared by Precursor-Directed Biosynthesis with Rhodococcus erythropolis
- 1.0348166 - MBÚ 2011 RIV US eng J - Journal Article
Schreiberová, O. - Krulikovská, T. - Sigler, Karel - Čejková, A. - Řezanka, Tomáš
RP-HPLC/MS-APCI Analysis of Branched Chain TAG Prepared by Precursor-Directed Biosynthesis with Rhodococcus erythropolis.
Lipids. Roč. 45, č. 8 (2010), s. 743-756. ISSN 0024-4201. E-ISSN 1558-9307
R&D Projects: GA MŠMT 2B08062
Institutional research plan: CEZ:AV0Z50200510
Keywords : Rhodococcus erythropolis * RP-HPLC/MS-APCI * Branched chain triacylglycerols
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.151, year: 2010
Reversed phase liquid chromatography–atmospheric pressure chemical ionization mass spectrometry (RP-HPLC/MS-APCI) was used to analyze both synthetic triacylglycerols (TAG) having 1–3 branched fatty acids (FA) in the molecule, and natural TAG prepared by precursor directed biosynthesis from valine, leucine and isoleucine and the corresponding branched short-chain acids in cultivations of Rhodococcus erythropolis. The technique made it possible to identify and quantify TAG differing in a single branched-chain FA. Altogether 11 TAG were synthesized, out of which 8 were synthesized stereospecifically
Permanent Link: http://hdl.handle.net/11104/0188764
Number of the records: 1