Gene Capture from Across the Grass Family in the Allohexaploid Elymus repens (L.) Gould (Poaceae, Triticeae) as Evidenced by ITS, GBSSI, and Molecular Cytogenetics

0348143 - BÚ 2011 RIV US eng J - Journal Article
Mahelka, Václav - Kopecký, David
Gene Capture from Across the Grass Family in the Allohexaploid Elymus repens (L.) Gould (Poaceae, Triticeae) as Evidenced by ITS, GBSSI, and Molecular Cytogenetics.
Molecular Biology and Evolution. Roč. 27, č. 6 (2010), s. 1370-1390. ISSN 0737-4038. E-ISSN 1537-1719
R&D Projects: GA ČR GP206/09/P312; GA ČR GA206/09/1126
Institutional research plan: CEZ:AV0Z60050516; CEZ:AV0Z50380511
Keywords : Elymus repens * hybridization * Triticeae
Subject RIV: EF - Botanics
Impact factor: 5.510, year: 2010

Allopolyploid origin of hexaploid grass Elymus repens from its native Central European distribution area was investigated using sequencing of multi-copy (ITS) and single-copy (GBSSI) DNA in concert with genomic and fluorescent in situ hybridization (GISH and FISH). The results suggested that there are at least six different lineages involved in the formation of the allpolyploid. While Pseudoroegneria and Hordeum represent the major genome constituents, the presence of further alien genetic material, originating from species from within (Taeniatherum and one unidentified donor) as well as outside the Triticeae (Panicum, Paniceae and Bromus, Bromeae), was revealed. The origin of E. repens most likely involved recent allopolyploidization in combination with old acquistions. Physical mapping of rDNA loci using FISH revealed that all rDNA loci except one minor were located on Pseudoroegneria-derived chromosomes, which suggests the loss of all Hordeum-derived loci but one.
Permanent Link: http://hdl.handle.net/11104/0188749