Senescence-Inducible Expression of Isopentenyl Transferase Extends Leaf Life, Increases Drought Stress Resistance and Alters Cytokinin Metabolism in Cassava

0348004 - ÚEB 2011 RIV GB eng J - Journal Article
Zhang, P. - Wang, W.Q. - Zhang, G. L. - Kamínek, Miroslav - Dobrev, Petre
Senescence-Inducible Expression of Isopentenyl Transferase Extends Leaf Life, Increases Drought Stress Resistance and Alters Cytokinin Metabolism in Cassava.
Journal of Integrative Plant Biology. Roč. 52, č. 7 (2010), s. 653-669. ISSN 1672-9072. E-ISSN 1744-7909
R&D Projects: GA MŠMT 1M06030; GA AV ČR IAA600380805
Institutional research plan: CEZ:AV0Z50380511
Keywords : MANIHOT-ESCULENTA CRANTZ * TRANSGENIC CASSAVA * AGROBACTERIUM-TUMEFACIENS
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.603, year: 2010

Cassava (Manihot esculenta Crantz) sheds its leaves during growth, especially within the tropical dry season. With the production of SAG12-IPT transgenic cassava we want to test the level of leaf retention and altered cytokinin metabolism of transgenic plants via the autoregulatory senescence inhibition system. After confirmation of transgene expression by molecular analysis and phenotype examination in greenhouse plants, two transgenic plant lines, 529-28 and 529-48, were chosen for further investigation. Detached mature leaves of 529-28 plants retained high levels of chlorophyll compared with wild-type leaves after dark-induced senescence treatment. Line 529-28 showed significant drought tolerance as indicated by stay-green capacity after drought stress treatment. Field experiments proved that leaf senescence syndrome was significantly delayed in 529-28 plants in comparison with wild-type and 529-48 plants.
Permanent Link: http://hdl.handle.net/11104/0188634