Feasibility of physical map construction from fingerprinted bacterial artificial chromosome libraries of polyploid plant species

0347998 - ÚEB 2011 RIV US eng J - Journal Article
Luo, M. C. - Ma, Y. Q. - You, F. M. - Anderson, O. D. - Kopecký, David - Šimková, Hana - Šafář, Jan - Doležel, Jaroslav - Gill, B. - McGuire, P. E. - Dvorak, J.
Feasibility of physical map construction from fingerprinted bacterial artificial chromosome libraries of polyploid plant species.
BMC Genomics. Roč. 11, č. 122 (2010), s. 1-8. ISSN 1471-2164. E-ISSN 1471-2164
Institutional research plan: CEZ:AV0Z50380511
Keywords : BREAD WHEAT * TETRAPLOID WHEAT * GENETIC-MAP
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.206, year: 2010

The presence of closely related genomes in polyploid species makes the assembly of total genomic sequence from shotgun sequence reads produced by the current sequencing platforms exceedingly difficult, if not impossible. Genomes of polyploid species could be sequenced following the ordered clone sequencing approach employing contigs of bacterial artificial chromosome (BAC) clones and BAC-based physical maps. In this work, physical mapping utilizing the SNaPshot HICF of BAC libraries of allohexaploid common wheat in which it is possible to construct single-chromosome or single-chromosome-arm BAC libraries from DNA of flow-sorted chromosomes and bypass the obstacles created by polyploidy. Physical maps of equally good BAC contigs, specific for three wheat single-chromosome libraries (3B, 3AS and 3DS), were constructed in this work. Because of the high purity of the resulting assembled contigs, they can be directly used for genome sequencing.
Permanent Link: http://hdl.handle.net/11104/0188629