Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection

0347925 - BC 2011 RIV GB eng J - Journal Article
Takasu, Yoko - Kobayashi, I. - Beumer, K. - Uchino, K. - Sezutsu, H. - Sajwan, S. - Carroll, D. - Tamura, T. - Žurovec, Michal
Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection.
Insect Biochemistry and Molecular Biology. Roč. 40, č. 10 (2010), s. 759-765. ISSN 0965-1748. E-ISSN 1879-0240
R&D Projects: GA AV ČR IAA500070601; GA ČR GAP305/10/2406; GA MŠMT(CZ) LC06077
Institutional research plan: CEZ:AV0Z50070508
Keywords : gene targeting * nonhomologous and joining * Lepidoptera
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.018, year: 2010

The absence of a general gene-targeting system for systematic reverse genetic studies has been a significant limitation in silkworm research. The authors performed direct zinc finger nuclease mRNA injection to /B. mori/ embryos and induced mutations in an epidermal color gene /BmBLOS2. /The results suggest that the /B. mori/ double-strand break repair system relies on microhomologies rather than on a ligase IV-dependent mechanism typical for /Drosophila/. The observed frequency of germline mutations was sufficient for future use in gene targeting relying solely on molecular methods of screening.
Permanent Link: http://hdl.handle.net/11104/0188585