Long-term pioglitazone treatment augments insulin sensitivity and PKC-epsilon and PKC-theta activation in skeletal muscles in sucrose fed rats

0347885 - FGÚ 2011 RIV CZ eng J - Journal Article
Marková, I. - Zídek, Václav - Musilová, Alena - Šimáková, Miroslava - Mlejnek, Petr - Kazdová, L. - Pravenec, Michal
Long-term pioglitazone treatment augments insulin sensitivity and PKC-epsilon and PKC-theta activation in skeletal muscles in sucrose fed rats.
Physiological Research. Roč. 59, č. 4 (2010), s. 509-516. ISSN 0862-8408. E-ISSN 1802-9973
R&D Projects: GA MŠMT(CZ) 1M0520; GA MŠMT(CZ) ME08006; GA AV ČR(CZ) IAA500110604; GA MZd(CZ) NR9387; GA MZd(CZ) NR9359; GA MZd(CZ) NS9759
Institutional research plan: CEZ:AV0Z50110509
Keywords : pioglitazone * PKC * insulin resistance
Subject RIV: FB - Endocrinology, Diabetology, Metabolism, Nutrition
Impact factor: 1.646, year: 2010

It has been suggested that thiazolidinediones (TZDs) ameliorate insulin resistance in muscle tissue by suppressing muscle lipid storage and the activity of novel protein kinase C (nPKC) isoforms. To test this hypothesis, we analyzed long-term metabolic effects of pioglitazone and the activation of nPKC-epsilon and -theta isoforms in an animal model of the metabolic syndrome, the spontaneously hypertensive rat (a congenic SHR strain with wild type Cd36 gene) fed a diet with 60 % sucrose from the age of 4 to 8 months. Pioglitazone-treated rats exhibited significantly increased membrane/total (cytosolic plus membrane) ratio of both PKC-epsilon and PKC-theta isoforms compared to untreated controls. These results suggest that amelioration of insulin resistance after long-term pioglitazone treatment is associated with increased activation of PKC-epsilon and -theta isoforms in spite of increased lipid concentration in skeletal muscles
Permanent Link: http://hdl.handle.net/11104/0188558