Autocrine effects of visfatin on hepatocyte sensitivity to insulin action

0347884 - FGÚ 2011 RIV CZ eng J - Journal Article
Škop, V. - Kontrová, K. - Zídek, Václav - Pravenec, Michal - Kazdová, L. - Mikulík, Karel - Sajdok, J. - Zídková, J.
Autocrine effects of visfatin on hepatocyte sensitivity to insulin action.
Physiological Research. Roč. 59, č. 4 (2010), s. 615-618. ISSN 0862-8408. E-ISSN 1802-9973
R&D Projects: GA AV ČR(CZ) IAA500110805; GA MŠMT(CZ) ME08006; GA MZd(CZ) NR9387; GA MZd(CZ) NR9359
Institutional research plan: CEZ:AV0Z50110509; CEZ:AV0Z50200510
Keywords : Visfatin * RNA interference * insulin mimetic effects
Subject RIV: FB - Endocrinology, Diabetology, Metabolism, Nutrition
Impact factor: 1.646, year: 2010

Visfatin was originally described as an adipokine with insulin mimetic effects. Since liver plays a major role in glucose metabolism, we studied visfatin effects on insulin-stimulated cellular glucose uptake in Fao rat hepatocytes using RNA interference (RNAi). RNAi-mediated downregulation of visfatin expression in Fao cells was associated with significantly reduced NAD biosynthesis (0.3+/-0.01 vs. 0.5+/-0.01 mmol/h/g, P<0.05) and with significantly decreased incremental glucose uptake after stimulation with insulin when compared to controls with normal expression of visfatin (0.6+/-0.2 vs. 2.2+/-0.5 nnmol/g/2 h, P=0.02). These results provide evidence that visfatin exhibits important autocrine effects on sensitivity of liver cells to insulin action possibly through its effects on NAD biosynthesis
Permanent Link: http://hdl.handle.net/11104/0188557