Number of the records: 1
Auxin influx inhibitors 1-NOA, 2-NOA, and CHPAA interfere with membrane dynamics in tobacco cells
- 1.0347619 - ÚEB 2011 RIV GB eng J - Journal Article
Laňková, Martina - Smith, R. S. - Pešek, Bedřich - Kubeš, Martin - Zažímalová, Eva - Petrášek, Jan - Hoyerová, Klára
Auxin influx inhibitors 1-NOA, 2-NOA, and CHPAA interfere with membrane dynamics in tobacco cells.
Journal of Experimental Botany. Roč. 61, č. 13 (2010), s. 3589-3598. ISSN 0022-0957. E-ISSN 1460-2431
R&D Projects: GA AV ČR KJB600380702; GA MŠMT(CZ) LC06034
Grant - others:_(CZ) CZ.2.16/3.1.00/21159
Institutional research plan: CEZ:AV0Z50380511
Keywords : Auxin efflux carrier * auxin influx carrier * auxin transport
Subject RIV: EF - Botanics
Impact factor: 4.818, year: 2010
Synthetic auxins that differ in the mechanisms of their transport across the plasma membrane together with polar auxin transport inhibitors have been used in many studies on particular auxin carriers and their role in plant development. In this report, the mechanism of action of the auxin influx inhibitors (1-naphthoxyacetic acid (1-NOA), 2-naphthoxyacetic acid (2-NOA), and 3-chloro-4-hydroxyphenylacetic acid (CHPAA)) is examined by direct measurements of auxin accumulation, cellular phenotypic analysis, as well as by localization studies of Arabidopsis thaliana auxin carriers heterologously expressed in Nicotiana tabacum BY-2 cell suspensions. The mode of action of 1-NOA, 2-NOA, and CHPAA has been shown to be linked with the dynamics of the plasma membrane. The most potent inhibitor, 1-NOA, blocked the activities of both auxin influx and efflux carriers, whereas 2-NOA and CHPAA at the same concentration preferentially inhibited auxin influx.
Permanent Link: http://hdl.handle.net/11104/0188363
File Download Size Commentary Version Access 2010_Lankova_JOURNAL OF EXPERIMENTAL BOTANY_3589.pdf 7 754.4 KB Other open-access
Number of the records: 1