Size determination of Acipenser ruthenus spermatozoa in different types of electron microscopy

0347391 - BC 2011 RIV GB eng J - Journal Article
Pšenička, M. - Tesařová, Martina - Těšitel, J. - Nebesářová, Jana
Size determination of Acipenser ruthenus spermatozoa in different types of electron microscopy.
Micron. Roč. 41, č. 5 (2010), s. 455-460. ISSN 0968-4328. E-ISSN 1878-4291
R&D Projects: GA AV ČR KAN200520704
Grant - others:GA ČR(CZ) GPP502/10/P426
Program: GP
Institutional research plan: CEZ:AV0Z60220518
Keywords : Scanning electron microscopy * Size determination * Sterlet spermatozoa * Sterlet spermatozoa
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.649, year: 2010

In this study three types of scanning electron microscopes were used for the size determination of spermatozoa of sterlet Acipenser ruthenus - high vacuum scanning electron microscope (SEM, JEOL 6300), environmental scanning electron microscope (ESEM, Quanta 200 FEG), field emission scanning electron microscope (FESEM, JEOL 7401F) with cryoattachment Alto 2500 (Gatan) and transmission electron microscope (TEM, JEOL 1010). The use of particular microscopes was tied with different specimen preparation techniques. The aim of this study was to evaluate to what degree the type of used electron microscope can influence the size of different parts of spermatozoa. Results confirmed that the classical chemical procedure of specimen preparation for SEM including critical point drying method led to a significant contraction of all measured values, which could deviate up to 30% in comparison with values measured on the fresh chemically untreated specimen in ESEM.
Permanent Link: http://hdl.handle.net/11104/0188179