In position 7 l- and d-Tic-substituted oxytocin and deamino oxytocin: NMR study and conformational insights

0347360 - ÚOCHB 2011 RIV AT eng J - Journal Article
Spyranti, Z. - Fragiadaki, M. - Magafa, V. - Borovičková, Lenka - Spyroulias, G. A. - Cordopatis, P. - Slaninová, Jiřina
In position 7 l- and d-Tic-substituted oxytocin and deamino oxytocin: NMR study and conformational insights.
Amino Acids. Roč. 39, č. 2 (2010), s. 539-548. ISSN 0939-4451. E-ISSN 1438-2199
Institutional research plan: CEZ:AV0Z40550506
Keywords : oxytocin analogues * deaminooxytocin * NMR
Subject RIV: CC - Organic Chemistry
Impact factor: 4.106, year: 2010

NMR study (NOESY, TOCSY, H-1-C-13 HSQC spectra) of analogues of oxytocin and deaminoocytocin having in position 7 L- or D-Tic is presented. The existence of both cis and trans configurations of the Cys(6)-D-Tic(7) bond is supported by observation of two sets of cross-peaks for H-1 and C-13 nuclei for most of the residues of the peptide not only in NOESY and TOCSY but also in H-1-C-13 HSQC spectra. The MS and HPLC indicate the presence of a single molecule/peptide, and NMR data thus suggest that this second set of peaks is due to the cis conformation.
Permanent Link: http://hdl.handle.net/11104/0188154