Benzimidazole resistance allele haplotype diversity in United Kingdom isolates of Teladorsagia circumcincta supports a hypothesis of multiple origins of resistance by recurrent mutation

0347281 - BC 2011 RIV AU eng J - Journal Article
Skuce, P. - Stenhouse, L. - Jackson, F. - Hypša, Václav - Gilleard, J.
Benzimidazole resistance allele haplotype diversity in United Kingdom isolates of Teladorsagia circumcincta supports a hypothesis of multiple origins of resistance by recurrent mutation.
International Journal for Parasitology. Roč. 40, č. 11 (2010), s. 1247-1255. ISSN 0020-7519. E-ISSN 1879-0135
Institutional research plan: CEZ:AV0Z60220518
Keywords : BZ resistance * b-Tubulin * Haplotype diversity * Haplotype diversity
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.822, year: 2010

Polymorphisms in the isotype 1 beta-tubulin gene are important genetic determinants of benzimidazole (BZ) resistance in a number of parasitic nematode species including Teladorsagia circumcincta, a major gastrointestinal nematode of sheep. This study investigates the genetic diversity at this locus in a BZ-resistant Isolate of T circumcincta (MTc15) derived from a sheep farm in UK that was open to animal, and therefore parasite, migration. Pyrosequencing was used to determine the frequency of single nucleotide polymorphisms (SNPs) known to be associated with BZ resistance. This was followed by a combination of single strand conformation polymorphism (SSCP) analysis and nucleotide sequencing to sample allelic diversity in a 276 bp fragment immediately surrounding the isotype I beta-tubulin F200Y mutation The genetic diversity at this locus was extremely high, with seven different haplotypes found to contain the resistant F200Y polymorphism in this single resistant isolate.
Permanent Link: http://hdl.handle.net/11104/0188090