Terminal restriction fragment length measurement errors are affected mainly by fragment length, G+C nucleotide content and secondary structure melting point

0347130 - BÚ 2011 RIV NL eng J - Journal Article
Bukovská, Petra - Jelínková, Markéta - Hršelová, Hana - Sýkorová, Zuzana - Gryndler, Milan
Terminal restriction fragment length measurement errors are affected mainly by fragment length, G+C nucleotide content and secondary structure melting point.
Journal of Microbiological Methods. Roč. 82, č. 3 (2010), s. 223-228. ISSN 0167-7012. E-ISSN 1872-8359
R&D Projects: GA MŠMT 1M0571
Institutional research plan: CEZ:AV0Z60050516; CEZ:AV0Z50200510
Keywords : T-RFLP * drift * DNA fragment
Subject RIV: EF - Botanics
Impact factor: 2.018, year: 2010

Several methods of molecular analysis of microbial diversity, including terminal restriction fragment length polymorphism (T-RFLP) analysis are based on measurement of the DNA fragment length. Significant variation between sequence-determined and measured length of restriction fragments (drift) has been observed, which can affect the efficiency of the identification of microorganisms in the analyzed communities. In this study, principal component analysis and multiple regression analysis revealed that secondary structure melting point and G+C nucleotide content, besides the fragment length, contribute to the variation observed, whereas the contribution of purine
Permanent Link: http://hdl.handle.net/11104/0187975