RECQ5 helicase associates with the C-terminal repeat domain of RNA polymerase II during productive elongation phase of transcription

0347094 - ÚMG 2011 RIV GB eng J - Journal Article
Kanagaraj, R. - Huehn, D. - Mackellar, A. - Menigatti, M. - Zheng, L. - Urban, Václav - Shevelev, Igor - Greenleaf, A.L. - Janščák, Pavel
RECQ5 helicase associates with the C-terminal repeat domain of RNA polymerase II during productive elongation phase of transcription.
Nucleic Acids Research. Roč. 38, č. 22 (2010), s. 8131-8140. ISSN 0305-1048. E-ISSN 1362-4962
R&D Projects: GA ČR GA204/09/0565
Grant - others:SNSF(CH) 3100A0-116008; NIH(US) GM040505
Institutional research plan: CEZ:AV0Z50520514
Keywords : RECQ5 DNA helicase * transcription * genome stability
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 7.836, year: 2010

It is known that transcription can induce DNA recombination, thus compromising genomic stability. RECQ5 DNA helicase promotes genomic stability by regulating homologous recombination. Recent studies have shown that RECQ5 forms a stable complex with RNA polymerase II (RNAPII) in human cells, but the cellular role of this association is not understood. Here, we provide evidence that RECQ5 specifically binds to the Ser2,5-phosphorylated C-terminal repeat domain (CTD) of the largest subunit of RNAPII, RPB1, by means of a Set2–Rpb1-interacting (SRI) motif located at the C-terminus of RECQ5. We also show that RECQ5 associates with RNAPII-transcribed genes in a manner dependent on the SRI motif. Notably, RECQ5 density on transcribed genes correlates with the density of Ser2-CTD phosphorylation, which is associated with the productive elongation phase of transcription.
Permanent Link: http://hdl.handle.net/11104/0187948