The transcription factor EGR1 regulates metastatic potential of v-src transformed sarcoma cells

0347089 - ÚMG 2011 RIV CH eng J - Journal Article
Čermák, Vladimír - Kosla, Jan - Plachý, Jiří - Trejbalová, Kateřina - Hejnar, Jiří - Dvořák, Michal
The transcription factor EGR1 regulates metastatic potential of v-src transformed sarcoma cells.
Cellular and Molecular Life Sciences. Roč. 67, č. 20 (2010), s. 3557-3568. ISSN 1420-682X. E-ISSN 1420-9071
R&D Projects: GA AV ČR KAN200520801; GA MŠMT(CZ) LC06061; GA ČR(CZ) GA204/07/1030
Institutional research plan: CEZ:AV0Z50520514
Keywords : smooth muscle cells * endothelial kinase * Rho kinase
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 7.047, year: 2010

To advance our understanding of metastatic cancer dissemination, we have developed a model system that is based on two v-src transformed chicken sarcoma cell lines-the highly metastatic parental PR9692 and a non-metastasizing but fully tumorigenic clonal derivative PR9692-E9. Oligonucleotide microarray analysis of both cell lines revealed that the gene encoding the transcription factor EGR1 was downregulated in the non-metastatic PR9692-E9 cells. Further investigation demonstrated that the introduction of exogenous EGR1 into PR9692-E9 cells restored their metastatic potential to a level indistinguishable from parental PR9692 cells. Microarray analysis of EGR1 reconstituted cells revealed the activation of genes that are crucial for actin cytoskeleton contractility (MYL9), filopodia formation (MYO10), the production of specific extracellular matrix components (HAS2, COL6A1-3) and other essential pro-metastatic abilities.
Permanent Link: http://hdl.handle.net/11104/0187946