Application of morphology filters to compensation of lateral illumination inhomogeneities in confocal microscopy images

0347023 - FGÚ 2011 RIV CZ eng C - Conference Paper (international conference)
Michálek, Jan - Čapek, Martin - Mao, X. W. - Kubínová, Lucie
Application of morphology filters to compensation of lateral illumination inhomogeneities in confocal microscopy images.
Analysis of biomedical signals and images. Brno: University of technology, 2010, s. 49-54. ISBN 978-80-214-4106-4. ISSN 1211-412X.
[International EURASIP Conference Biosignal /20./. Brno (CZ), 27.06.2010-29.06.2010]
R&D Projects: GA ČR(CZ) GA102/08/0691; GA ČR(CZ) GA304/09/0733; GA MŠMT(CZ) LC06063; GA MŠMT(CZ) MEB090910
Institutional research plan: CEZ:AV0Z50110509
Keywords : CLSM * illumination inhomogeneities
Subject RIV: JD - Computer Applications, Robotics

Due to multiple distortion effects, confocal laser scanning microscopy (CLSM) images of even homogeneous specimen regions suffer from irregular brightness variations. In this paper, a fast correction method based on estimating a spatially variable illumination gain, and multiplying acquired CLSM images by the inverse of the estimated gain, is presented. The method does not require any special calibration (reference) images since the gain estimate is extracted from the CLSM image being corrected itself. The proposed approach exploits two types of morphological filters: the median filter and a morphological operator called the upper Lipschitz cover
Permanent Link: http://hdl.handle.net/11104/0187897