Inhibition of 19S proteasomal regulatory complex subunit PSMD8 increases polyspermy during porcine fertilization in vitro

0346866 - BTÚ 2011 RIV IE eng J - Journal Article
Yi, Y.J. - Manandhar, G. - Sutovsky, M. - Jonáková, Věra - Park, Ch.S. - Sutovsky, P.
Inhibition of 19S proteasomal regulatory complex subunit PSMD8 increases polyspermy during porcine fertilization in vitro.
Journal of Reproductive Immunology. Roč. 84, č. 2 (2010), s. 154-163. ISSN 0165-0378. E-ISSN 1872-7603
R&D Projects: GA MŠMT(CZ) 1M06011; GA ČR(CZ) GA303/09/1285
Institutional research plan: CEZ:AV0Z50520701
Keywords : Sperm * Proteasome * Fertilization * Zona pellucida * Acrosome
Subject RIV: EC - Immunology
Impact factor: 2.204, year: 2010

The 26S proteoasome is composed of a 20S core with substrate degradation activity, and a 19S regulatory complex that acts in substrate recognition, deubiquitination, priming and transport to the 20S core. The study examined the role of PSMD8, a subunit of the 19S complex, in sperm-ZP penetration during porcine fertilization in vitro (IVF). Immunofluorescence localized PSMD8 to the outer acrosomal membrane, acrosomal matrix and the inner acrosomal membrane. Subunit PSMD8 co-immunoprecipitated with acrosomal surface-associated spermadhesin AQN1. This association indicates that the sperm acrosome-borne proteasomes become exposed onto the sperm surface following the acrosomal exocytosis. Since immunological blocking of subunit PSMD8 increases the rate of polyspermy during porcine fertilization, the activity of the 19S complex may be a rate-limiting factor contributing to anti-polyspermy defense during porcine fertilization.
Permanent Link: http://hdl.handle.net/11104/0187781