Interference with the 19S proteasomal regulatory complex subunit PSMD4 on the sperm surface inhibits sperm-zona pellucida penetration during porcine fertilization

0346865 - BTÚ 2011 RIV DE eng J - Journal Article
Yi, Y.J. - Manandhar, G. - Sutovky, M. - Zimmerman, S.W. - Jonáková, Věra - Leeuwen van, F.W. - Oko, R. - Park, Ch.S. - Sutovsky, P.
Interference with the 19S proteasomal regulatory complex subunit PSMD4 on the sperm surface inhibits sperm-zona pellucida penetration during porcine fertilization.
Cell and Tissue Research. Roč. 341, č. 2 (2010), s. 325-340. ISSN 0302-766X. E-ISSN 1432-0878
R&D Projects: GA MŠMT(CZ) 1M06011; GA ČR(CZ) GA303/09/1285; GA MZd(CZ) NS10009
Institutional research plan: CEZ:AV0Z50520701
Keywords : Sperm * Proteasome * Fertilization * Zona pellucida * Acrosome * Alzheimer's * Porcine
Subject RIV: CE - Biochemistry
Impact factor: 2.804, year: 2010

Proteolysis of ubiquitinated sperm and oocyte proteins by the 26S proteasome is necessary for mammalian fertilization. The study examined the role of PSMD4, a subunit of 19S regulatory complex responsible for proteasome-substrate recognition, in sperm-ZP penetration during porcine fertilization in vitro (IVF). Immunofluorescence localized PSMD4 in the sperm acrosome. Immunoprecipitation and proteomic analysis revealed that PSMD4 co-precipitated with porcine sperm-associated acrosin inhibitor (AI). Ubiquitinated species of AI were isolated from boar sperm extracts by affinity purification of ubiquitinated proteins. Some proteasomes appeared to be anchored to the sperm head inner acrosomal membrane. In conclusion, the 19S regulatory complex subunit PSMD4 is involved in the sperm-ZP penetration during fertilization. The recognition of substrates on the ZP by the 19S proteasomal regulatory complex is essential for porcine/mammalian fertilization in vitro.
Permanent Link: http://hdl.handle.net/11104/0187780